RASHTRASANT TUKADOJI MAHARAJ NAGPUR UNIVERSITY

Course Name

Objectives

CELL BIOLOGY (PROKARYOTES AND EUKARYOTES)

· Elucidate the cellular organization of the eukaryotic and prokaryotic cells

· Explain cell cycle and signal transduction pathways

MOLECULAR BIOLOGY I

· Know the gene function, genomic organization

· Know the process of DNA replication, transcription and translation in detail

Elective 1: GENETICS – I

· Elucidate the genetic basis of inheritance and gene interaction

· Explain the chemical nature of the gene and importance of epigenetics.

Elective 1: INDUSTRIAL AND ENVIRONMENTAL BIOTECHNOLOGY- I

· Explain the steps involved in complete Bioprocess engineering.

· Explain how to manufacture the different cell lines and their use in biopharmaceuticals.

· Interpret the effect of environmental pollution on climate change and biological system.

· Elucidate about the environment and its beneficiation using genetically engineered organisms

RESEARCH METHODOLOGY AND BIOSTATISTICS

· Prepare the objectives and types of research. Apply inclusion, exclusion criteria and blinded trials with respect to clinical trial.

· Prepare the components of thesis writing. Distinguish between thesis components and journal components

· Apply the methods of sampling, diagrammatic and graphical representation of data for analyzing the data.

· Use the measure of central tendency, deviation, correlation and regression for analysing and to inferring data. Illustrate on probability and theoretical distribution of data and also outline the hypothesis testing

PRACTICAL 1

· Sterilize microbial growth media, culture the bacteria and measure their growth, stain them and identify them microscopically

· Perform various immunotechniques use for research and pathology.

Isolate DNA, RNA and check their purity.

PRACTICAL 2

· Solve the problems related to basic Mendelian genetics, Non Mendelian genetics and population genetics

· Isolate industrially important microbes, develop bioreactors and isolate the related material.

· Develop biofertilizers, biopesticides and perform environmental monitoring for biological and physical factors

Course Name

Objectives

BIOCHEMISTRY

· Explain the structure and function of biomolecules like carbohydrates, lipids, proteins.

· Elucidate the metabolism of carbohydrate, lipids and amino acids and vitamins.

· Interpret the importance of enzymes and its regulation.

MOLECULAR BIOLOGY II

· Explain the different Recombination and horizontal gene transfer methods.

· Know the DNA damage repair mechanisms.

· Explain the gene expression regulation mechanism.

· Explain Molecular genetics in Humans

Elective 2: GENETICS – II

· Elucidate the Importance of Population, Evolutionary, Developmental and behavioral genetics.

· Learn the concept of Plant breeding, Eugenetics and chromosome mapping in humans.

Elective 2: INDUSTRIAL AND ENVIRONMENTAL BIOTECHNOLOGY- II

· Explain about different types of Bioreactors and their control of bioprocess parameters.

· Explain about the different steps involved in development of Bioprocess.

· Explain about different Downstream processing steps required for final protein product formation.

· Explain about the Industrial scale production of different type of chemicals and their modifications.

PRACTICAL 3

· Estimate protein, carbohydrate, lipid content in the sample using biochemical methods and perform experimentations related to enzymology.

· Learn to file patent, develop entrepreneurship or startup idea, and understand the process of biosafety assessment of GMOs in India.

· Search various databases related to Nucleic acid and protein according to the need of analysis, Analyze sequences, align sequences and generate phylogenic tree.

· Perform spectrometric, chromatographic and microscopic techniques.

PRACTICAL 4

· Perform Micropropagation, haploid production and various plant tissue culture methods

· Prepare media for animal cell culture, culturing cells and checking viability

· Performing in vitro recombination using bacterial DNA.

On Job Training

· The objective of on-job training/field project is to allow the student to gain vocational training in academics/ research/industry based on plants and allied organisms. It is also aimed to encourage the student to take-up a life-time vocation based on the program he/she is pursuing. On-job training/field work will also allow the student to work in team and gain experience, which will be helpful in his/her future life

Course Name

Objectives

GENETIC ENGINEERING AND NANOTECHNOLOGY

· Explain the importance of different Molecular tools used in Genetic Engineering.

· Explain the importance of DNA, RNA and protein synthesis along with the hybridization and sequencing methods.

· Explain the characterization of nanomaterials and applications of Nanotechnology.

· Explain the Bioethics relevant to Indian context.

BIOINFORMATICS AND Biophysical Techniques

· Explain the importance of bioinformatics databases, tools and resources.

· Explain the use of Bioinformatics to analyses the biological data.

· Explain the use and applications of Microscopy and spectrometry techniques.

· Explain the applications of Biophysical techniques in industries and research laboratories

PLANT AND ANIMAL TISSUE CULTURE

· Learn the basic plant tissue culture techniques from different sources.

· Learn the concept of Transgenic plants production.

· Explain the Animal tissue culture, its advanced culture techniques and applications.

· Explain the applications of Animal Biotechnology.

Elective 3: MOLECULAR DIAGNOSTICS – I

· Explain about different gene and signal amplification techniques.

· Explain the basic and advanced nucleic acid sequencing methods.

· Explain about the molecular diagnostics methods and their use in different disease conditions.

· Explain about the different models used in molecular diagnosis.

Elective 3:

PLANT GENETIC ENGINEERING I

· Elucidate techniques of plant breeding, Suspension culture, Haploid plant production, Protoplast culture and other plant tissue culture techniques.

· Explain the different Plant transformation technology and its applications.

· Interpret the Molecular marker aided breeding, metabolic engineering and industrial production.

· Elucidate techniques of plant breeding, Suspension culture, Haploid plant production, Protoplast culture and other plant tissue culture techniques.

PRACTICAL 5

MOLECULAR DIAGNOSTICS

· Amplify, isolate and identify specific gene and proteins related to specific disease.

· Perform bioethical survey and analyze the data for output.

· Analyzing immunohistographs using software and analysis done using cancer database for prognosis and survival.

PRACTICAL 5

PLANT GENETIC ENGINEERING

· Perform Micropropagation, haploid production of Plants

· Isolate, quantitatively and qualitatively analyze plant DNA

· Perform various plant tissue culture methods and their callus induction

· Transgene formation using genetic engineering

Minor Project

· The objective of research project is to train the student in identifying the problem of research, develop the hypothesis, design the experiments/surveys to test the hypothesis, collect & analyze the data and draw conclusions from it. In addition, the aim is also to prepare the student to present the data in various forms such as project report, presentation in conferences & seminars and research paper. Research project is also aimed to prepare the student for doctoral research after the completion of the program.

Course Name

Objectives

RECOMBINANT DNA TECHNOLOGY

· Learn about the recombinant DNA technology and its use in DNA modification and gene cloning

· Learn different techniques used for RDT

· Perform in vitro recombination using bacterial DNA

IMMUNOLOGY

· Learn to differentiate between innate and adaptive immune system

· Understand the immunological response.

· Learn about the immune system, antigen representation and immune cell activation

· Explain various immunological techniques

IPR, ENTERPRENEURSHIP, BIOSAFETY, QA AND QC

· Understand the basics of patent application filing and the applications of IPR in India and abroad and learn to file the patent application.

· Understand the issues and concerns related to biotechnological inventions for filing patents

· Get motivated for entrepreneurship development.

· Understand the procedure for biosafety and QA QC rules

Elective 4: MOLECULAR DIAGNOSTICS – II

· Understand the biology of the diseases- infection caused by bacteria, virus, fungi, protozoa etc.

· Understand different genetic disorders and its diagnosis.

· Understand different metabolic disorders and its diagnosis.

· Understand different Plant diseases, its diagnosis and methods to control the diseases caused by different pathogens.

Elective 4:

PLANT GENETIC ENGINEERING II

· Elucidate gene Cloning, Somatic Embryogenesis and in vitro Pollination and fertilization.

· Interpret the Genetic resources, germplasm conservation and production of secondary metabolites through cell culture technique and the genetic engineering for crop improvement

Major Project

· The student will have to carry out a research-based project work in the third and fourth semester. The project work may be carried out in the parent department or any other institute. For this the student will be attached to any of the national/regional/private research institute/organization for the duration of the third and fourth semester. If the student is working in the organization other than the parent department, then it will be the responsibility of the student to attend the classes and other departmental activities in order to be eligible to appear for the examination.